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  4. Rat extracellular superoxide dismutase [Cu-Zn](Cu/Zn-SOD/SOD3)ELISA kit

Rat extracellular superoxide dismutase [Cu-Zn](Cu/Zn-SOD/SOD3)ELISA kit

  • 中文名稱：
    大鼠胞外銅鋅超氧化物歧化酶(Cu/Zn-SOD/SOD3)酶聯免疫試劑盒
  • 貨號：
    CSB-E14981r
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    大鼠胞外銅鋅超氧化物歧化酶(Cu/Zn-SOD/SOD3)酶聯免疫試劑盒（CSB-E14981r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的SOD3含量。SOD3即超氧化物歧化酶 3，它是一種細胞外抗氧化酶，能將超氧陰離子轉化為過氧化氫和氧氣。在研究機制方面，其在多種疾病如心血管疾病、肺部疾病等中發揮作用，可調節氧化應激水平，有望成為治療相關疾病的潛在靶點。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于研究氧化應激相關疾病模型、藥物干預對SOD3活性的影響，以及細胞或組織微環境中抗氧化機制的動態變化?？蓾M足神經退行性疾病、心血管疾病、炎癥反應等研究領域中對SOD3蛋白表達水平的定量分析需求，為探索氧化還原平衡調控機制提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Sod3 ELISA Kit; Sod-3 ELISA Kit; Extracellular superoxide dismutase [Cu-Zn] ELISA Kit; EC-SOD ELISA Kit; EC 1.15.1.1 ELISA Kit; Superoxide dismutase B ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat Cu/Zn-SOD/SOD3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:200 Average % 93  
    Range % 89-95  
    1:400 Average % 104  
    Range % 100-107  
    1:800 Average % 95  
    Range % 90-97  
    1:1600 Average % 98  
    Range % 91-101  
  • 回收率：
    The recovery of rat Cu/Zn-SOD/SOD3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 89-97  
    EDTA plasma (n=4) 95 92-99  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    1000 2.644 2.588 2.616 2.461  
    500 2.264 2.344 2.304 2.149  
    250 1.650 1.632 1.641 1.486  
    125 1.044 1.028 1.036 0.881  
    62.5 0.624 0.636 0.630 0.475  
    31.2 0.383 0.368 0.376 0.221  
    15.6 0.248 0.239 0.244 0.089  
    0 0.156 0.154 0.155 　  
  • 數據處理：
  • 貨期：
    3-5 working days

引用文獻

產品評價

靶點詳情

  • 功能：
    Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen.
  • 基因功能參考文獻：
    1. Data show that aging is associated with reduction in superoxide dismutase (SOD) Cu/Zn-SOD protein expression and total SOD enzymatic activity in mesenteric lymphatic vessel (MLV). PMID: 22540739
    2. The impact of SOD3 on Akt and Erk1/2 cell survival pathways in rat hind limb injury model, is reported. PMID: 21909393
    3. ER stress leads to the down-regulation of claudin-5 among tight junction proteins and may induce the elevation of endothelial permeability and leakage of EC-SOD into the vitreous body. PMID: 21736484
    4. These data are important for understanding the pathogenesis of vascular diseases in which the synthesis of endothelial NO is decreased or extracellular O2- production exceeds the functional capacity of SOD3. PMID: 20033309
    5. Furin proteolytically processes the heparin-binding region of extracellular superoxide dismutase PMID: 11861638
    6. Levels are not significantly altered in the rat prostate during aging and thus is unlikely to be an important factor in the evolution of epithelial cell hyperplasia PMID: 16372329
    7. EcSOD, CuZnSOD, catalase, and MMP-2 mRNA expression did not statistically vary between aortic aneurysm and normal tissue PMID: 17196988
    8. SOD3 minimizes O2- levels preserving NO availability at resting conditions. SOD3 promotes NO mediated vasodilatation by scavenging O2- and basal SOD3 levels is able to inactivate O2- produced by 100% oxygen breathing preserving vasodilator effect of NO. PMID: 19198181
    9. SOD3 has a selective anti-inflammatory role in ischemic damages preventing the migration of reactive oxygen producing monocyte/macrophages PMID: 19495415

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  • 亞細胞定位：
    Secreted, extracellular space. Golgi apparatus, trans-Golgi network.
  • 蛋白家族：
    Cu-Zn superoxide dismutase family
  • 數據庫鏈接：


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